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  1. How does the polarity of TLC work in separating compounds?

    Feb 7, 2025 · The polarity of TLC (thin-layer chromatography) is important because it helps determine how well compounds will separate during the chromatography process.

  2. Why we need a co-spot on a TLC plate? - Answers

    Jun 19, 2025 · A co-spot on a TLC (thin-layer chromatography) plate is essential for comparison purposes. It allows for the direct visual comparison between the sample and a known standard, …

  3. What is TLC? - Motorcycle Forum

    Jul 4, 2008 · I was looking at a used bike and it said "1986, does not run, Kerker exhaust, great starter bike needs t.l.c." What does the TLC mean?

  4. Why is ninhydrin used in amino analysis in TLC? - Answers

    May 23, 2024 · Ninhydrin is used in amino acid TLC because it readily stains amino acids by reacting with the amine groups. This reaction takes place very quickly and creates a brownish-color that can …

  5. Why polar compounds have less Rf value? - Answers

    Jan 19, 2025 · The non-polar solvent system will have less affinity for the polar silica gel in the TLC plate, allowing the acetophenone to move further up the plate during the chromatography process.

  6. Why sand and MgSO4 added during chromatography? - Answers

    Jun 4, 2024 · Why is sand added when performing thin layer chromatography? Sand is added when performing thin layer chromatography (TLC) to create a stable and uniform support for the TLC plate.

  7. Why is it important not to let your fingers touch a TLC plate?

    May 21, 2024 · The polarity of a TLC plate is important in chromatography because it affects how different compounds move and separate on the plate.

  8. Why is it necessary to cover the developing chamber tightly ... - Answers

    Jun 3, 2024 · Covering the developing chamber tightly is important because it creates a saturated atmosphere, which allows for consistent and even solvent movement up the chromatography plate. …

  9. Why do some compounds travel further on TLC plates than others?

    May 24, 2024 · it doesn't depends upon the TLC plates.. as it depends upon the solvents in which these compounds migrate.

  10. Why it is necessary to spray the chromatography with ninhydrin?

    Jun 28, 2024 · Spraying a chromatography with resorcinol is necessary to visualize the spots of separated compounds, particularly in thin-layer chromatography (TLC).