🤔How to surface sterilize plant tissues for tissue culture? ☘️ Surface sterilization of explants (tissue or plant part used to regenerate a whole plant in vitro) is necessary to produce disease-free plants in tissue culture. 👩🏻‍🔬 A range of surface sterilization techniques has been developed to clean explants for in vitro propagation. They involve chemicals like: 🧪 Sodium hypochlorite (also known as bleach) 🧪 Ethanol 🧪 Calcium Hypochlorite 🧪 And, some rare ones include mercuric chloride
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